荧光素酶
生物发光
生物发光成像
体内
荧光素
临床前影像学
细胞
化学
报告基因
生物
分子成像
癌症研究
体外
病理
细胞生物学
生物化学
医学
转染
生物技术
基因
作者
Susan A. Brutkiewicz,Marc S. Mendonca,Keith M. Stantz,Kathleen Comerford,Robert M. Bigsby,Gary D. Hutchins,Mark G. Goebl,Maureen A. Harrington
出处
期刊:Luminescence
[Wiley]
日期:2007-02-07
卷期号:22 (3): 221-228
被引量:34
摘要
Abstract In vivo bioluminescence imaging is becoming a very important tool for the study of a variety of cellular and molecular events or disease processes in living systems. In vivo bioluminescence imaging is based on the detection of light emitted from within an animal. The light is generated as a product of the luciferase–luciferin reaction taking place in a cell. In this study, we implanted mice with tumour cells expressing either a high or a low level of luciferase. In vivo bioluminescence imaging was used to follow tumour progression. Repeated luciferin injection and imaging of high and low luciferase‐expressing tumours was performed. While low luciferase‐expressing tumours grew similarly to vector controls, growth of the high luciferase‐expressing tumours was severely inhibited. The observation that a high level of luciferase expression will inhibit tumour cell growth when an animal is subjected to serial in vivo bioluminescence imaging is potentially an important factor in designing these types of studies. Copyright © 2007 John Wiley & Sons, Ltd.
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