巨噬细胞移动抑制因子
鼻咽癌
川地163
肿瘤相关巨噬细胞
组织微阵列
爱泼斯坦-巴尔病毒
肿瘤微环境
川地68
巨噬细胞
巨噬细胞极化
CD11c公司
M2巨噬细胞
肿瘤浸润淋巴细胞
生物
免疫学
癌症研究
病毒
病理
医学
免疫系统
细胞因子
免疫疗法
免疫组织化学
内科学
放射治疗
体外
表型
基因
生物化学
作者
Guofei Feng,Yifei Xu,Ning Ma,Kaoru Midorikawa,Shinji Oikawa,Hatasu Kobayashi,Satoshi Nakamura,Hajime Ishinaga,Zhe Zhang,Guangwu Huang,Kazuhiko Takeuchi,Mariko Murata
出处
期刊:BMC Cancer
[BioMed Central]
日期:2021-08-18
卷期号:21 (1)
被引量:8
标识
DOI:10.1186/s12885-021-08675-x
摘要
To assess the effects of Epstein-Barr virus (EBV) and human papillomavirus (HPV) infection on the tumor microenvironment, we examined the relationship between viral infection status, macrophage migration inhibitory factor (MIF), and tumor-associated macrophages in nasopharyngeal carcinoma (NPC).A tissue microarray containing 150 cores from 90 patients with NPC and six with chronic inflammation was used. EBV and HPV status were detected using in situ hybridization with commercial EBER1 and HPV16/18 probes. Immunofluorescence double staining of MIF, pan-macrophage marker CD68, M1 macrophage marker CD11c, and M2 macrophage marker CD163 were analyzed using the same tissue microarray. The levels of these markers between NPC and inflammation cases and between tumor nests and stroma were compared. Correlations among these markers were analyzed.We found EBER1(+) cases in 90% of NPC patients, including 10% EBV/HPV co-infection. M1 macrophages mainly infiltrated the tumor nest, while M2 macrophages infiltrated the tumor stroma. We found a significant positive correlation between EBER1 levels and MIF levels in tumor nests and a significant positive correlation between HPV16/18 and CD11c(+) cell levels in NPC tissues.It is suggested that MIF is associated with EBV, and M1 macrophage infiltration is affected by HPV status in NPC.
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