生物传感器
双金属
费斯特共振能量转移
荧光
检出限
化学
猝灭(荧光)
荧光团
线性范围
分析化学(期刊)
光化学
色谱法
生物化学
物理化学
量子力学
物理
作者
Yawen Wu,Xiaolong Chen,Changjun Hou,Peng Lü,Ping Lan,Jiawei Li,Mei Yang,Danqun Huo
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:147 (5): 862-869
被引量:4
摘要
Human epidermal growth factor receptor 2 (HER2) is one of the specific markers of breast cancer, which is of great significance to the early diagnosis and prognosis of breast cancer. Here, a fluorescence biosensor was established to detect HER2 based on the fluorescence resonance energy transfer (FRET) and photoinduced electron transfer (PET) occurring between the bimetal-polydopamine organic framework with core-shell structure Au@PDA@UiO-66 and the Cy5 fluorophore in HER2-Cy5-Apt. Au@PDA@UiO-66 owns high-efficiency fluorescence quenching ability due to its large specific surface area and strong adsorption of single-stranded DNA. When the target appears, the fluorescence recovery space mediated by the target is large, so the proposed biosensor has better sensitivity in theory. Under optimized conditions, the proposed fluorescent biosensor can detect HER2 in a range of 0.005 ng mL-1 to 15 ng mL-1, with an actual detection limit as low as 0.005 ng mL-1. Corresponding selective experiments, reproducible experiments, and spiked experiments performed well, showing its great potential in HER2 detection.
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