Cloning and Expression Analysis of Cyclin Gene(CsCYC1) of Tea Plant

The full length cDNA of cyclin gene(CsCYC1) was cloned from the sprouting buds of tea plant(Camellia sinensis) by SMART-RACE-PCR based on the partial sequences in sprouting bud SSH-cDNA library.The sequence and structure characteristics of its encoded protein were analyzed,and its expression patterns during the time of bud dormancy and bud break were evaluated by quantitative real-time PCR(qRT-PCR).The full length cDNA is 1956 bp(GenBank Accession No.JF795471),which includes 1 320 bp ORF and encodes 439 amino acid residues with a putative molecular mass of 49.35 kD.CsCYC1 has a typical conserved cyclin-box domain near the C-terminus and 3D structure of cyclin family.Phylogenetic analysis showed that CsCYC1 had 77%,74%,72%,68% and 67% similarity with cyclin of Vitis vinifera,Ricinus communis,Populus tomentosa,Arabidopsis lyrata and Arabidopsis thaliana,respectively.The results of quantitative real-time PCR showed that the expression level of CsCYC1 at dormant stages was lower than that at growth stages,and the highest expression level emerged at sprouting stage.It indicated that CsCYC1 had very close relationship with tea plant budbreak in spring.