Determination of Hydroxyproline in sea cucumber with DABS-Cl pre-column derivatization by high performance liquid chromatography

The study is meant to determine the content of hydroxyproline in sea cucumber. After hydrolysis, the sample was decomposed into amino acids mixture, including hydroxyproline. The amino acids were derived with DABS-Cl. Liquid chromatographic separations were performed on a C18 column with a citric acid buffer-acetonitrile gradient elution program. UV detector was used to determine the hydroxyproline at 436 nm wavelength. The results indicated that hydroxyproline can be well separated from other substances. The linear range was from 40-2 000 μg·L-1 (r0.999). The recoveries of spiked samples were 84.2%-104%, and the RSD was less than 10%. The proposed method is sensitive, accurate and suitable for the determination of hydroxyproline in sea cucumber.