The nanocrystalline TiO_2 film,which is even,transparent and porous,was prepared through homogenous precipitation methods on the surface of ceramics and glasses.The photocatalytic mechanism,reactive oxygen species' forming mechanism, and TiO_2 particle's bactericidal mechanism of this semiconductive nanocrystalline film were investigated.The bactericidal capability of the film was examined with the methods of GB15979-1995.The results showed that the film has the wide sterilizing range,high sterilizing speed and sterilizing efficiency.Its antibacterial rates to Staphylococcus Aureus,Escherichia Coli and Candida Albicans in 30 min are excess of 90.00%.The killing rate to HBV in 20 min reaches to 43.43%,and it maybe has certain bactericidal effects on respiratory tract virus such as flu virus,SARS etc.