Analysis of genetic detecting in 1202 outpatients with suspected herpes simplex virus infection

Objective To investigate the situation of patients with genital suspected herpes simplex virus(HSV) infection in Xintang,Guangzhou.Methods To test herpes simplex virus(HSV-I/II) of 1202 cases of outpatients by fluorescence quantitative polymerase chain reaction technology(FQ-PCR).Results The positive rate of 1202 casas of outpatients were 0.67%(8/1202) with HSV-I and 23.46%(282/1202) with HSV-II.The total positive rate of HSV infection were 24.13%(290/1202).There was significant difference between the positive detection rate of HSV-I and HSV-II(P0.01).The positive rate of HSV in male and female patients were 24.62%(193/748) and 23.21%(97/418),respectively.There was no significant difference of the positive detection rate between male and female(P0.05).Conclusion ① The detection rate of HSV in the outpatients with suspected genital herpes HSV infection was high in Xintang area,which nearly a quarter.The infection rate was difference in different types of HSV,and the main type was HSV-II.The result illustrated that clinical diagnosis should pay attention to detect HSV in outpatients with suspected genital herpes virus in order to avoid misdiagnosis.② There was no difference of HSV infection between male and female.③ The features of FQ-PCR were simplicity,high sensitivity,fast in detection of HSV and it is worth for clinical application.