The Effects of Ammonia-N Stress on the Activities of ATPase,ACP and AKP in Different Tissues and Organs of Mud Crab Scylla paramamosain

A experimental ecology method was conducted to investigate the effects of ammonia-N(0 mg/L(control),10 mg/L,20 mg/L,30 mg/L,40 mg/L) stress on the activities of ATPase,ACP and AKP in gill,hepatopancreas and muscle of mud crab Scylla paramamosain to ammonia-N exposure for 0,24,48,72 and 96 h.The results showed that Na+-K+-ATPase activity in gill of S.paramamosain in C10 group and C20 group decreased at 24 h,and increased at 48,72 and 96 h with ammonia-N concentration increasing(p0.05),while the Na+-K+-ATPase activity in the gill of S.paramamosain in C30 group and C40 group was consistently decreased during experimental time;Ca2+-Mg2+-ATPase activity in the gill of S.paramamosain in C40 group consistently increased with ammonia-N stress time prolonging.ACP activity in gill and hepatopancreas of S.paramamosain decreased significantly with ammonia-N concentration increasing at 48,72,96 h(p0.05),while the ACP activity in muscle of S.paramamosain decreased significantly with ammonia-N concentration increasing and ammonia-N stress time prolonging(p0.05);AKP activity in gill and hepatopancreas of S.paramamosain decreased significantly with ammonia-N concentration increasing(p0.05),while the AKP activity in muscle of S.paramamosain increased significantly with ammonia concentration increasing after 24 h ammonia stress(p0.05).Thus,ammonia-N stress significantly affected the physiological parameter of S.paramamosian.