Analysis of HSP70 protein polymorphism with 2-DE and Western-blot

Aim: To analysis the HSP70 protein polymorphism with 2-DE technique. Methods: The protein components of 5 volunteers' lymphocytes were analyzed with 2-DE,and the HSP70 components were identified with the specific antibody using Western blot.Results: With 2-DE and Western blot, at least 5 subtypes of HSP70 could be detected. Conclusion: The subtypes of HSP70 can be well separated using 2-DE and Western blot, and the key steps of 2-DE can be optimized.