Effects of Fluorescent Wavelength on the Resolution of Two-photon Fluorescence Confocal Imaging

The analytic expressions of the fluorescent intensity are derived in two_photon fluorescence confocal imaging. The numerical results show that the transverse resolution, the axial resolution, and the intensity of the spot increase as the rate of fluorescent wavelength to illustrated wavelength increases. The resolution in two_photon fluorescence confocal microscopy is higher than that in single_photon fluorescence confocal microscopy.