Determination of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 in Rupixiao Granule by HPLC

OBJECTIVE To establish an HPLC method for determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Rupixiao granule.METHODS The chromatographic conditions included C18 column(CAPCELL PAK,150 mm×4.6 mm,5 μm),acetonitrile as mobile phase A,water as mobile phase B.The flow rate was 1.0 mL.min-1.The detection wavelength was 203 nm.Gradient elution: 0.12 min,A: 19%,B: 81%;12-60 min,A:19%→36%,B: 81→64%.RESULES The retention time of notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Rb1 was 21,25,51 min,respectively.The resolution was above 1.5.The linear range of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 was 0.100 2-2.004 μg,0.418 8?8.376 μg and 0.187-3.74 μg.The average recovery was 98.5%,99.6% and 96.5%,RSD was 1.9%,1.3% and 1.9%,respectively.CONCLUSION The method is simple,accurate and reproduciable.It can be used for routine analysis of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Rupixiao granule simultaneously.