The influence of oxidative stress on the level of malondialdehyde (MDA) in different areas of the rabbit brain.

Lipid peroxidation is a process which is determined by the extent of peroxide-forming free radicals mechanism and the peroxide-removing antioxidant system. To assess the peroxidation process, products of lipid peroxidation are analysed. Products of lipid peroxidation, such as thiobarbituric acid (TBA) are defined as thiobarbituric acid reactive material. In the present study we measured lipid peroxidation in three areas of the rabbit brain: brain hemispheres, brainstem and cerebellum. Supernatant and 10% homogenate were used to determine the level of malondialdehyde (MDA) according to the Ohkawa method. We measured levels of MDA before and after oxidative stress. Our results showed an important role of oxidative stress, because levels of MDA were increased significantly in comparison to the level of control, in all different areas of the rabbit brain.