益生菌
肠道菌群
胶质增生
中枢神经系统
免疫学
肠道通透性
失调
病理生理学
内科学
生物
炎症
医学
内分泌学
神经科学
遗传学
细菌
作者
Harpreet Kaur,Kumi Nagamoto-Combs,Svetlana A. Golovko,Mikhail Y. Golovko,Marilyn G. Klug,Colin K. Combs
标识
DOI:10.1016/j.neurobiolaging.2020.04.009
摘要
Evidence suggests that changes in intestinal microbiota may affect the central nervous system. However, it is unclear whether alteration of intestinal microbiota affects progression of Alzheimer’s disease (AD). To understand this, wild-type control (C57BL/6) mice were compared with the AppNL-G-F model of disease. We used probiotic supplementation to manipulate the gut microbiota. Fecal samples were collected for microbiota profiling. To study brain and intestinal inflammation, biochemical and histological analyses were performed. Altered metabolic pathways were examined by quantifying eicosanoid and bile acid profiles in the brain and serum using ultraperformance liquid chromatography–tandem mass spectrometry. We observed that brain pathology was associated with intestinal dysbiosis and increased intestinal inflammation and leakiness in AppNL-G-F mice. Probiotic supplementation significantly decreased intestinal inflammation and gut permeability with minimal effect on amyloid-β, cytokine, or gliosis levels in the brain. Concentrations of several bile acids and prostaglandins were altered in the serum and brain because of AD or probiotic supplementation. Our study characterizes intestinal dysfunction in an AD mouse model and the potential of probiotic intervention to ameliorate this condition.
科研通智能强力驱动
Strongly Powered by AbleSci AI