肝细胞癌
癌基因
免疫印迹
癌症研究
下调和上调
小RNA
报告基因
细胞生长
医学
癌症
生物
细胞周期
基因表达
基因
内科学
生物化学
遗传学
作者
Jianxing Zheng,Daming Cheng,Dongyang Wu,Libing Wang,Fengzhi Qu,Xiaotang Wu,Lan Cheng,Yanbin Wei,Xiaogang Liu
出处
期刊:Personalized Medicine
[Future Medicine]
日期:2021-03-01
卷期号:18 (2): 97-106
被引量:15
标识
DOI:10.2217/pme-2020-0027
摘要
Objective: This study explored the potential function of miR-452-5p in hepatocellular carcinoma (HCC) and clarified the mechanism underlying HCC progression. Materials & methods: Real-time quantitative PCR was used to detect miR-452-5p and COLEC10 mRNA expression in HCC, western blot was performed to test COLEC10 protein expression. The regulatory mechanism of miR-452-5p/COLEC10 in HCC cells was explored using CCK-8, wound healing assay, Transwell and dual-luciferase reporter assay. Results: MiR-452-5p was greatly upregulated in HCC cells, and it served as an oncogene playing an active role in HCC cell proliferation, migration and invasion. COLEC10 was identified as the target of miR-452-5p in HCC attenuating the promoting effect of miR-452-5p on HCC cells upon overexpression. Conclusion: MiR-452-5p can promote the progression of HCC via targeting COLEC10.
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