The ameliorative effect ofAspergillus awamori on aflatoxin B1-induced hepatic damage in rabbits

This study was conducted to investigate the effect of dietary supplementation of Aspergillus awamori on aflatoxin B 1 (AFB 1 )-induced liver damage in rabbits. Administration of AFB 1 (0.3 mg/kg diet) led to a significant reduction in body weight, body weight gain, total feed intake, total serum proteins, albumin, high density lipoprotein-cholesterol, phagocytic activity, phagocytic index, and the antioxidant enzyme, glutathione peroxidase (GPx). Moreover, AFB 1 administration was associated with a significant increase in feed conversion ratio, lipid peroxidation and serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase, lactate dehydrogenase and total bilirubin. In addition, livers of AFB 1 -supplemented animals showed fatty degeneration with vacuolisation, focal areas of necrosis, mononuclear cells infiltration hyperplasia of bile ducts and sinusoids. A significant increase in the hepatic expression of the biotransformation gene ( Cyp3A6 ), stress-sensitive genes ( HO1 and SOD1 ), and inflammation-related genes ( IL6, TNFa, NF-kB, and Cox2 ) was also observed. Supplementation of the diets with 0.05, 0.1 or 0.15% A. awamori ameliorated all AFB 1 deleterious effects with the best improvement observed at the lowest concentration. This is the first investigation to report that supplementation of rabbit diets with A. awamori has an ameliorative effect against AFB 1 -induced liver damage possibly through preventing hepatic oxidative stress, promoting the antioxidant defence systems, and inhibiting expression of Cyp3A6 , HO1 , SOD1 , IL6, TNFa, NF-kB, and Cox2 . Therefore, A. awamori could be used as a potential preventive or therapeutic agent for aflatoxicosis.