低温保存
外周血单个核细胞
免疫系统
男科
活力测定
免疫学
化学
低温生物学
医学
生物
细胞
细胞生物学
体外
生物化学
胚胎
作者
Yan Xu,Qiongna Zou,Frankliu Gao,Daimeng Wang,Suxia Xue,Hebei Lin,Hao Guo,Xiaoyu He,Huanfeng Yang,Dayong Gao
出处
期刊:Biopreservation and Biobanking
[Mary Ann Liebert]
日期:2021-08-01
卷期号:19 (4): 318-323
被引量:5
标识
DOI:10.1089/bio.2020.0058
摘要
It is well known that the warming process is a critical step in cell cryopreservation, affecting the survival rate of the cryopreserved cells. However, there is a lack of understanding and optimization of the warming process for the cryopreserved human peripheral blood mononuclear cells (PBMCs) that are greatly needed for the cellular/immune therapies worldwide. In this study, the effect of the warming process on cryosurvival of the PBMCs was investigated, resulting in a recommendation of an optimal warming method. In the experiments, all PBMC samples were cooled by a fixed slow cooling process and stored in a liquid nitrogen tank. The frozen samples were then warmed in water baths with stirring at various temperatures, 37°C, 42°C, and 65°C, respectively. After thawing, PBMC's viability as well as phenotypic and functional analyses were performed and evaluated. It was shown that a relatively rapid warming process at 65°C in a water bath with stirring generated a significant improvement of cell viability, recovery, and functionality of the cryopreserved PBMCs. In addition, interferon-γ and interleukin-2 secretion were much higher in PBMCs thawed at 65°C than that in 42°C and 37°C, respectively. This study suggests that a rapid warming process at 65°C in a water bath should be used to replace the current conventional warming approach at 37°C.
科研通智能强力驱动
Strongly Powered by AbleSci AI