Abstract 13: High-Throughput, Non-Invasive Raman Spectroscopy of Anticancer Nanomedicine for Liver Cancer Therapeutic Intervention

Abstract Purpose: Liver cancer remains one of the most difficult to treat, heterogeneous forms of cancer with poor prognosis. Available current therapies face many limitations. Raman spectroscopy is a promising platform for clinical drug development and surgical interventions for improved patient outcomes. Herein, we present a new insight with a rapid, non-destructive, non-invasive Raman spectroscopy modality based molecular signatures unique to anticancer nanomedicine signifying distinct stratification in liver cancer treated tissues of SCID mice. Methods: The therapeutic intervention was investigated using a high-throughput Raman spectroscopy in ex vivo liver tissues treated with combinatorials; targeted and non-targeted polymeric nanocomplexes encapsulated microtubule inhibitors against metastatic liver cancer in SCID mice. Principal Component Analysis (PCA), Linear Discriminate Analysis (LDA) for multivariate data analysis, and cross validation method was used to extrapolate the high throughput Raman based treatment efficiency study in ex vivo liver tissues. Results: Raman spectral signatures obtained by Raman spectroscopy of liver tissue samples of healthy normal liver versus liver cancer cohorts exhibited complete discrimination of healthy ones from the affected diseased counterparts with high accuracy classification. This could be useful as a liver cancer diagnosis tool. Peculiar Raman profiles with distinct stratification of targeted anticancer nanomedicine combinatorials treated cohort were observed. Interestingly, apoptotic lipid bodies and amide I was the prominent features revealed in anticancer nanomedicine combinatorials treatment cohorts. Conclusion: Our approach is a significant advancement for unique Raman molecular signatures-based targeted anticancer nanomedicine combinatorials therapeutic intervention for liver cancer in tissues. Clinical translation of this proposed modality would define Raman based rapid, high-throughput platform for the clinical drug development and monitoring against liver cancer remains warranted. Citation Format: Radhika Poojari, Mithila Bhujbal, Arti Hole, Murali Krishna Chilakapati. High-Throughput, Non-Invasive Raman Spectroscopy of Anticancer Nanomedicine for Liver Cancer Therapeutic Intervention [abstract]. In: Proceedings of the 9th Annual Symposium on Global Cancer Research; Global Cancer Research and Control: Looking Back and Charting a Path Forward; 2021 Mar 10-11. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2021;30(7 Suppl):Abstract nr 13.