基质凝胶
间充质
间充质干细胞
基底膜
层粘连蛋白
庆大霉素保护试验
细胞外基质
体外
细胞生物学
细胞
基质(化学分析)
转移
上皮-间质转换
化学
生物
病理
医学
癌症
生物化学
遗传学
色谱法
作者
Erin M Bell,Marcia L Graves,Pamela M Dean,Tate O Goodman,Calvin D Roskelley
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 79-99
标识
DOI:10.1007/978-1-0716-2376-3_8
摘要
AbstractUnderstanding the modes and mechanisms of tumor cell invasion is key to developing targeted therapies against metastatic disease. In vitro assays modeling tumor progression have primarily been optimized for studying classical single-cell migration through an epithelial–mesenchymal transition (EMT). Although experimental and clinical histopathological evidence has revealed that tumor invasion is plastic and that epithelial carcinomas can invade by a range of modes that vary from single, mesenchyme-like cells, all the way to cohesive, collective units, few in vitro assays have been designed to assess these modes specifically. Thus, we have developed a Matrigel–Collagen I overlay assay that is suitable for identifying and quantifying both collective and mesenchymal invasion. This three-dimensional (3D) culture assay utilizes the features of Matrigel and Collagen I to mimic the laminin-rich basement membrane and the stiff, fibrillar Collagen I tumor microenvironment allowing for spheroid invasion to be assessed at the interface between these two matrix components.Key wordsTumor invasion3D CultureMatrigelCollagen IEMTCollective invasion
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