乳克鲁维酵母
异源的
克鲁维酵母
酵母
生物
异源表达
生物技术
工业生物技术
发酵
重组DNA
计算生物学
基因
酿酒酵母
生物化学
作者
Sebastian C. Spohner,Vivienne Schaum,Hendrich Quitmann,Peter Czermak
标识
DOI:10.1016/j.jbiotec.2016.02.023
摘要
Kluyveromyces lactis has emerged as one of the most important yeast species for research and industrial biotechnology. This Crabtree-negative species is suitable for the production of metabolites and heterologous proteins, and its ability to achieve high levels of protein secretion makes it an attractive alternative for industrial protein production. Since 1991, almost 100 recombinant proteins have been expressed in K. lactis, 20% of which have been produced in the last 2 years. This review provides an overview of the genetic modifications used to accomplish heterologous gene expression in K. lactis, as well as fermentation techniques, and recent examples of industrial proteins produced in this species.
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