Quality control method for commercially available wild Jujube leaf tea based on HPLC characteristic fingerprint analysis of flavonoid compounds

Journal of Separation ScienceVolume 37, Issue 1-2 p. 45-52 Research Article Quality control method for commercially available wild Jujube leaf tea based on HPLC characteristic fingerprint analysis of flavonoid compounds Rutan Zhang, Rutan Zhang Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, ChinaSearch for more papers by this authorJunhui Chen, Corresponding Author Junhui Chen Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China Correspondence: Dr. Junhui Chen, QingDao Key Lab on Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography of SOA, 6# Xian xia ling Road, Qingdao 266061, P. R. China E-mail:[email protected] Fax: +86-532-88963253Search for more papers by this authorQian Shi, Qian Shi Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, ChinaSearch for more papers by this authorZhaoyong Li, Zhaoyong Li Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, ChinaSearch for more papers by this authorZhengyun Peng, Zhengyun Peng Qingdao Wanlijiang Tea Co. Ltd, Qingdao, ChinaSearch for more papers by this authorLi Zheng, Li Zheng Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, ChinaSearch for more papers by this authorXiaoru Wang, Xiaoru Wang Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China Xiamen Huaxia Vocational College, Xiamen, ChinaSearch for more papers by this author Rutan Zhang, Rutan Zhang Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, ChinaSearch for more papers by this authorJunhui Chen, Corresponding Author Junhui Chen Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China Correspondence: Dr. Junhui Chen, QingDao Key Lab on Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography of SOA, 6# Xian xia ling Road, Qingdao 266061, P. R. China E-mail:[email protected] Fax: +86-532-88963253Search for more papers by this authorQian Shi, Qian Shi Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, ChinaSearch for more papers by this authorZhaoyong Li, Zhaoyong Li Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, ChinaSearch for more papers by this authorZhengyun Peng, Zhengyun Peng Qingdao Wanlijiang Tea Co. Ltd, Qingdao, ChinaSearch for more papers by this authorLi Zheng, Li Zheng Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, ChinaSearch for more papers by this authorXiaoru Wang, Xiaoru Wang Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China Xiamen Huaxia Vocational College, Xiamen, ChinaSearch for more papers by this author First published: 26 October 2013 https://doi.org/10.1002/jssc.201300841Citations: 29 Colour Online: See the article online to view Figs. 1 and 5 in colour. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Flavonoids are the main active components of natural medicinal plants with many physiological functions. In this study, an HPLC fingerprinting method based on the distribution and relative amount of 11 bioactive flavonoids was established for the quality evaluation of commercially available wild Jujube leaf tea (JLT) from China. Separation of the crude flavonoid extract was achieved on a column filled with C18 material with a high carbon content. The flavonoids in wild JLT were identified based on UV spectroscopy and accurate mass measurements by TOF-MS. Twenty-one batches of practical samples collected from different habitats were analyzed by using the developed HPLC method to construct the HPLC characteristic fingerprint of wild JLT. Then, combined with clustering and similarity analyses, the HPLC characteristic fingerprint was used for the authentication and quality evaluation of commercial wild JLT. Results indicated that the proposed HPLC characteristic fingerprint reflected the inherent characteristics of wild JLT collected from different regions. Authenticity identification and quality control of commercially available wild Jujube tea were achieved based on the HPLC characteristic fingerprint analysis. This new approach to bioactive component profiling provided a promising reference method for the quality evaluation of commercial wild flower and plant tea. Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description jssc3534-sup-0001-Suppmat.doc4.4 MB Table S1. Contents of rutin and other flavonoids in 21 JLT samples Figure S1. Effect of different types of C18 columns on the separation of flavonoids in JLT: A, Alltima C18 column; B, Agilent Eclipse XDB-C18 column; C, Agilent ZORBAX SB-C18 column; and D, Kromasil 100-5 C18 column Figure S2. Effect of the initial proportion of acetonitrile in the moblile phase on the separation of rutin from its isomeride (A:16%; B:18%) Figure S3. Effects of different extraction conditions on the extraction efficiency of rutin in JLT Figure S4. UV absorption spectrum (A), ESI-TOF/MS spectrum and measured isotopic mass distributions (B), and formula confirmation (C) of peak 4 Figure S5. Total ion chromatograms obtained in positive mode (A) and negative mode (B) of wild jujube leaf tea (No 1-11 are same as Figure S1 and Figure 2.) Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. 5 References 1 Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China, China Medical Science Press, Beijing 2010, pp. 343–344. 2Zhao, X. H., Yi, L. N., Lishizhen. Med. Mater. Med. Res. 2009, 20, 463–464. 3Wang, S. Y., Liu, Q. F., Qin, M. Z., Chin. Tradit. Pat. Med. 2000, 22, 794–796. 4Wu, S. X., Zhang, J. X., Chin. J. Chin. Mater. Med. 1993, 18, 685–687. 5Guo, S., Duan, J. A., Tang, Y. P., Qian, Y. F., Zhao, J. L. Qian, D. W., Su, S. 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