T细胞受体
CD8型
克隆(Java方法)
生物
细胞毒性T细胞
抗原
分子生物学
人类白细胞抗原
T细胞
T淋巴细胞
CTL公司*
过继性细胞移植
体外
免疫学
病毒学
基因
免疫系统
遗传学
作者
Timothy M. Clay,Mary Custer,Jessica Sachs,Patrick Hwu,Steven A. Rosenberg,Michael I. Nishimura
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:1999-07-01
卷期号:163 (1): 507-513
被引量:359
标识
DOI:10.4049/jimmunol.163.1.507
摘要
The tumor-associated-Ag MART-1 is expressed by most human melanomas. The genes encoding an alphabeta TCR from a MART-1-specific, HLA-A2-restricted, human T cell clone have been efficiently transferred and expressed in human PBL. These retrovirally transduced PBL cultures were MART-1 peptide reactive, and most cultures recognized HLA-A2+ melanoma lines. Limiting dilution clones were generated from three bulk transduced PBL cultures to investigate the function of individual clones within the transduced cultures. Twenty-nine of 29 CD8+ clones specifically secreted IFN-gamma in response to T2 cells pulsed with MART-1(27-35) peptide, and 23 of 29 specifically secreted IFN-gamma in response to HLA-A2+ melanoma lines. Additionally, 23 of 29 CD8+ clones lysed T2 cells pulsed with the MART-1(27-35) peptide and 15 of 29 lysed the HLA-A2+ melanoma line 888. CD4+ clones specifically secreted IFN-gamma in response to T2 cells pulsed with the MART-1(27-35) peptide. TCR gene transfer to patient PBL can produce CTL with anti-tumor reactivity in vitro and could potentially offer a treatment for patients with metastatic melanoma. This approach could also be applied to the treatment of other tumors and viral infections. Additionally, TCR gene transfer offers unique opportunities to study the fate of adoptively transferred T cells in vivo.
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