化学
肝星状细胞
量子点
荧光
荧光团
流式细胞术
生物物理学
冰冻切片程序
免疫荧光
纳米技术
抗体
分子生物学
病理
光学
材料科学
免疫学
医学
物理
生物
作者
Yi He,Kai Cheng,Zi‐Tao Zhong,Xiaolin Hou,Chang-Zhi An,Jing Zhang,Wei Chen,Bo Liu,Jing Yuan,Yuan‐Di Zhao
标识
DOI:10.1016/j.aca.2023.341210
摘要
The targeted labeling imaging of stellate cells on liver frozen section by immunofluorescence is a very promising visualization technique to study the distribution of stellate cells in the liver. In this study, water soluble carbon quantum dots that can emit blue, green and yellow fluorescence are synthesized by the hydrothermal method, and their sizes are 3.2, 3.7, and 4.3 nm, respectively. The three carbon quantum dots have good fluorescence stability, and the quantum yields are 36.1%, 26.3% and 21%, respectively. When the mass fraction of KCl in the blue carbon quantum dot dispersion system is 13%, it still maintains the liquid state at −30 °C. The final fluorescent probe is obtained after the carbon quantum dots are coupled with the secondary antibody, spectral characterizations confirm that the conjugate probe still maintains protein immunoactivity and has good stability. Cell experiments prove that the probe has good biocompatibility, the rabbit anti-mouse Desmin antibody is used as the primary antibody, the results of cellular immunofluorescence imaging and flow cytometry show that the probe can specifically label hepatic stellate cell at −20 °C. The results of liver frozen section experiments show that hepatic stellate cell can be specifically targeted and labeled by the fluorescent probe. This labeling technology provides an important technical means for elucidating the structure and function of the liver at the cellular level, exploring the liver pathological change, and designing and developing drug.
科研通智能强力驱动
Strongly Powered by AbleSci AI