A programmable multichannel chemiluminescence immunoassay sensor for automatic quantification of the total amount of fumonisin B1, B2 and B3

A programmable multichannel chemiluminescence immunoassay sensor was developed based on the biotin-streptavidin system for the automatic quantification of the total amount of fumonisin B₁, B₂ and B₃ in maize samples. First, the key parameters, such as the reaction time and reagent dosage were optimized to improve the sensitivity. All reagents were subsequently added to the corresponding wells of the reagent strips, which were further sealed to create a total fumonisin quantification kit. Finally, six samples were automatically and simultaneously analyzed within 20 min with the aid of a fully automated mycotoxin immunoassay analyzer. The limit of detection of this method is 16.32 μg/kg, with cross-reactivity for eight common mycotoxins being less than 0.66 %, and the spiking recovery rates ranging from 89.7 % to 103.7 %. In conclusion, the proposed programmable multichannel chemiluminescent immunoassay sensor provides a reliable, sensitive and practical detection tool for automated and high-throughput detection of FBs.