转移
癌症研究
上皮-间质转换
肿瘤微环境
趋化因子
癌症
蛋白激酶B
白细胞介素8
顺铂
生物
细胞迁移
细胞
细胞因子
信号转导
医学
免疫学
炎症
内科学
化疗
细胞生物学
肿瘤细胞
遗传学
作者
Swarali Joshi,Ritu Pandey,Ashok Kumar,Vikas Gupta,Neha Arya
出处
期刊:Cytokine
[Elsevier]
日期:2023-06-01
卷期号:166: 156155-156155
被引量:2
标识
DOI:10.1016/j.cyto.2023.156155
摘要
The tumor microenvironment plays a significant role in tumor growth, metastasis and chemoresistance via dysregulated signaling pathways. Toward this, an inflammatory chemokine, interleukin-8 (IL-8), is overexpressed in various cancers and is involved in tumor progression and chemoresistance. However, the mechanistic role of IL-8 in mediating metastasis and chemoresistance in oral squamous cell carcinoma (OSCC) is not known. In the present study, we evaluated the effect of IL-8 in regulating metastasis as well as chemoresistance in OSCC cell lines. For this, IL-8 was blocked exogenously using neutralizing IL-8 monoclonal antibody and IL-8 levels were enhanced by exogenous supply of recombinant human IL-8 (rhIL-8) to OSCC cells. The epithelial-to-mesenchymal transition (EMT) was evaluated using qPCR, migration by scratch/wound healing assay and invasion ability using transwell assay. rIL-8 induced chemoresistance was studied by apoptosis assay and the nuclear localization of NFκB using immunocytochemistry. IL-8 was significantly overexpressed in OSCC patients and cell lines. While exogenous blockade of IL-8 significantly reduced EMT, migration and invasion potential in OSCC cells, IL-8 overexpression upregulated these cellular traits thereby confirming the role of IL-8 in OSCC metastasis. Exogenous blockade of IL-8 also reversed chemoresistance in cisplatin resistant OSCC subline via NFκB signaling. IL-8 plays a crucial role in OSCC metastasis and its targeted blockade can help in management of cisplatin resistance.
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