绒毛尿囊膜
移植
卵泡发生
毛囊
生物
卵泡
保持生育能力
卵子发生
血管生成
卵巢
低温保存
细胞生物学
胚胎
医学
内科学
内分泌学
癌症研究
生育率
人口
环境卫生
作者
Camille Hossay,Luciana Cacciottola,Sarah Storder,Olivier Van Kerk,Marie-Madeleine Dolmans
摘要
Ovarian tissue cryopreservation and transplantation is an effective strategy for preserving fertility but has one major drawback, namely massive follicle loss occurring shortly after reimplantation due to abnormal follicle activation and death. Rodents are benchmark models for investigating follicle activation, but the cost, time, and ethical considerations are becoming increasingly prohibitive, thus driving the development of alternatives. The chick chorioallantoic membrane (CAM) model is particularly attractive, being inexpensive and maintaining natural immunodeficiency up to day 17 postfertilization, making it ideal to study short-term xenografting of human ovarian tissue. The CAM is also highly vascularized and has been widely used as a model to explore angiogenesis. This gives it a remarkable advantage over in vitro models and allows the investigation of mechanisms affecting the early post-grafting follicle loss process. The protocol outlined herein aims to describe the development of a CAM xenografting model for human ovarian tissue, with specific insights into the effectiveness of the technique, the graft revascularization time frame, and the tissue viability across a 6 day grafting period.
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