Ginsenoside F1 preparation from ginsenosides Re and Rg1 mixture of ginseng leaves by a 6-O-glycoside-ginsenosidase from Aspergillus sp. g383 strain

Ginsenoside F1 was prepared from ginseng-leaf ginsenosides Re and Rg1 using a 6-O-glycoside-ginsenosidase from Aspergillus sp.g383 strain. From 500 g ginseng leaves, a 25.1 g mixture of 54.2% Re and 45.8% Rg1 were extracted. The enzyme molecular weight was 54.5 kDa; the optimal temperature was 45 °C; and optimal pH, 5.0; Re's V max and Km were 11.6 mM/h and 10.9 mM, respectively; Rg1's were 7.69 mM and 0.917 mM/h. The 6-O-glycoside-ginsenosidase conversion rates of Re and Rg1 into F1 were faster than those of the other enzymes. In the preparation of ginsenoside F1, 2.5% mixture of Re and Rg1 from ginseng leaf-part was reacted at 45 °C for 36 h by crude enzyme at a low cost. From a 25 g mixture of Re and Rg1, 13.8 g ginsenoside F1 was obtained. The purity of F1 was 95%, and the molar yield from Re and Rg1 was 75%. F1 weight-yield from ginseng-leaves was 2.76%. Furthermore, ginseng-leaves were used to obtain extra ginsrnoside Re and ginseng-leaf PPD-ginsenosides. Thus, the ginsenosides F1, which could be used safely in ginseng food, cosmetics or drugs, were successfully produced by a non-GMO crude-enzyme from a Re and Rg1 mixture of ginseng-leaves.