双歧杆菌
长双歧杆菌
双歧杆菌
封堵器
生物
脂多糖
毛螺菌科
微生物学
肠粘膜
粘蛋白
益生菌
免疫学
乳酸菌
紧密连接
生物化学
内科学
细菌
医学
厚壁菌
16S核糖体RNA
基因
发酵
遗传学
作者
Yingxue Yue,Yuqi Wang,Qinggang Xie,Xiuli Lv,Linyi Zhou,Etareri Evivie Smith,Ting Cao,Yifan Zhang,Bailiang Li,Guicheng Huo,Weiwei Ma
标识
DOI:10.1021/acs.jafc.3c00421
摘要
Changes in the functions of the intestinal barrier occur in parallel with the development of sepsis. The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS). The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells. The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group. In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α). The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids. Furthermore, the BLBB group leads to higher levels of deoxycholic acid and biosynthesized linoleate. This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota. Bifidobacterium bifidum E3 combined with Bifidobacterium longum subsp. infantis E4 may thus serve as a probiotic against the intestinal injury caused by LPS.
科研通智能强力驱动
Strongly Powered by AbleSci AI