肌痛
塔克曼
冠状病毒
病毒学
医学
肺炎
实时聚合酶链反应
病毒
喉部
呼吸道
腹痛
呕吐
病毒性肺炎
2019年冠状病毒病(COVID-19)
呼吸系统
内科学
胃肠病学
疾病
生物
基因
传染病(医学专业)
外科
生物化学
作者
Morteza BAGHERI,Tooba Hallaj,Legha Ansari,Firouz Ghaderi PAKDEL
出处
期刊:Mædica
[TARUS Media]
日期:2023-09-15
卷期号:18 (3)
标识
DOI:10.26574/maedica.2023.18.3.442
摘要
Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is known as a positivesense single-strand RNA virus and leads to Coronavirus disease 2019 .Coronaviruses significantly impact the human respiratory tract.Coronavirus disease is potentially fatal and transmissible in the world.In this study we evaluated the presence or absence of SARS-CoV-2 in 220 patients with un-explained pneumonia by TaqMan real-time PCR assay regarding open reading frame (ORF1ab) and nucleocapsid (N) protein genes.Materials and methods: Totally, 224 patients entered the study.Upper and lower respiratory tract secretion samples were obtained during 2020 from patients.Samples contained nose and throat swabs with viral transport medium.RNA was isolated from clinical samples with the GenePure Plus fully automatic Nucleic Acid Purification System, Hangzhou, China).Outcomes: 72.32% of cases were positive for COVID-19.All positive cases had the most common symptoms of illness regarding fatigue, dry cough, dyspnea, headache, abdominal pain, nausa, vomiting and myalgia.Fever was observed in 50% of positive cases.Chest computed tomography (CT) scan of all tested patients indicated two-sided chest involvement.Conclusions: Detection of COVID-19 by TaqMan real-time PCR seems to be a powerful method for the screening and detection of novel corona virus infection.
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