Carrier-free Nano-Tetrandrine (PURE-T) for Psoriasis Treatment: Significant Therapeutic Efficacy and Its Regulatory Mechanism on Stratum Corneum Inflammation

As an incurable systemic autoimmune disease, psoriasis is in urgent need of safe and effective treatment strategies. As a natural product extracted from Chinese herbs, Tetrandrine (Tet) has a wide range of pharmacological effects. In this study, we developed a carrier-free nano Tet topical formulation (PURE-T) by antisolvent method, and verified that it could selectively attenuate the toxicity of HaCaT cells in the normal state and potentiate the efficacy of HaCaT cells in the inflammatory state in vitro, and in vivo experiments verified that it could normalize the epidermal layer of the skin of imiquimod (IMQ)-induced psoriasis-like mice, and reduce the infiltration of inflammatory cells in the superficial layer of the dermis; mechanistic studies suggested that PURE-T may achieve therapeutic treatment of psoriasis through the GSDMC-mediated noninflammatory pyroptosis pathway. This safe, effective, carrier-free topical nanoformulation has potential for clinical translation and large-scale production.