Encapsulation of resveratrol within size-controlled nanoliposomes: Impact on solubility, stability, cellular permeability, and oral bioavailability

This study examined the influence of the nanoliposomes (LPs) particle size on the solubility, antioxidant stability, in vitro release profile, Caco-2 cellular transport activity, cellular antioxidant activity, and in vivo oral bioavailability of resveratrol (RSV). LPs with sizes of 300, 150, and 75 nm were prepared using the thin-lipid film hydration method, followed by ultrasonication for 0, 2, and 10 min, respectively. Formulating small LPs (< 100 nm) was effective to enhance the solubility, in vitro release profile, cellular permeability, and cellular antioxidant activity of RSV. A similar pattern was observed for in vivo oral bioavailability. However, the size reduction of RSV-loaded LPs did not promote the antioxidant stability of RSV, owing to their large surface area used to interact with harsh environments. This study provides the better understanding of the appropriate particle size range of LPs to improve their in vitro and in vivo performances of RSV as an effective carrier for oral administration.