In Vivo Electrochemical Biosensing Technologies for Neurochemicals: Recent Advances in Electrochemical Sensors and Devices

In vivo electrochemical sensing of neurotransmitters, neuromodulators, and metabolites plays a critical role in real-time monitoring of various physiological or psychological processes in the central nervous system. Currently, advanced electrochemical biosensors and technologies have been emerging as prominent ways to meet the surging requirements of in vivo monitoring of neurotransmitters and neuromodulators ranging from single cells to brain slices, even the entire brain. This review introduces the fundamental working principles and summarizes the achievements of in vivo electrochemical biosensing technologies including voltammetry, amperometry, potentiometry, field-effect transistor (FET), and organic electrochemical transistor (OECT). According to the elaborate feature of sensing technology, versatile strategies have been devoted to solve critical issues associated with the sensing of neurochemicals under an intricate physiological environment. Voltammetry is a universal technique to investigate electrochemical processes in complex matrices which could realize the miniaturization of electrodes, while amperometry serves as a well-suited approach offering high temporal resolution which is favorable for the fast oxidation-reduction kinetics of neurochemicals. Potentiometry realizes quantitative analysis by recording the potential difference with reduced invasiveness and high compatibility. FET and OECT serve as amplification strategies with higher sensitivity than traditional technologies. Furthermore, we point out the current shortcomings and address the challenges and perspectives of in vivo electrochemical biosensing technologies.