免疫荧光
H&E染色
数字化病理学
组织病理学
病理
组织学
人工智能
医学
计算机科学
免疫组织化学
放射科
抗体
免疫学
作者
Jia-Ren Lin,Yuan Chen,Daniel Campton,Jeremy Cooper,Shannon Coy,Clarence Yapp,Juliann B. Tefft,Erin McCarty,Keith L. Ligon,Scott J. Rodig,Steven Reese,Tad George,Sandro Santagata,Peter K. Sorger
出处
期刊:Nature cancer
[Springer Nature]
日期:2023-06-22
卷期号:4 (7): 1036-1052
被引量:15
标识
DOI:10.1038/s43018-023-00576-1
摘要
Precision medicine is critically dependent on better methods for diagnosing and staging disease and predicting drug response. Histopathology using hematoxylin and eosin (H&E)-stained tissue (not genomics) remains the primary diagnostic method in cancer. Recently developed highly multiplexed tissue imaging methods promise to enhance research studies and clinical practice with precise, spatially resolved single-cell data. Here, we describe the 'Orion' platform for collecting H&E and high-plex immunofluorescence images from the same cells in a whole-slide format suitable for diagnosis. Using a retrospective cohort of 74 colorectal cancer resections, we show that immunofluorescence and H&E images provide human experts and machine learning algorithms with complementary information that can be used to generate interpretable, multiplexed image-based models predictive of progression-free survival. Combining models of immune infiltration and tumor-intrinsic features achieves a 10- to 20-fold discrimination between rapid and slow (or no) progression, demonstrating the ability of multimodal tissue imaging to generate high-performance biomarkers.
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