Hepatic Response in Cytochrome P450 and De-alkylase Activity of Olive Flounder (Paralichthys olivaceus) Exposed to Water-borne Phenanthrene

Olive flounder (Paralichthys olivaceus) was exposed to water-borne phenanthrene for 4 weeks. After the exposure for 2-weeks, hepatic cytochrome P450 contents were significantly elevated. Induction of hepatic ethoxy resorufin-O-deethylase (EROD) activity was significantly increased in flounders treated with 1.0 and 2.0 μM phenanthrene, compared to untreated group and 0.5 μM treated group. However, there were no significant changes in pentoxyresorufin-O-deethylase (PROD) activity in hepatic microsome of all the phenanthrene-treated groups, compared to the untreated group. Phenanthrene has the potential to induce cytochrome P450 and EROD enzyme of the olive flounder.