Construction of the Seed-specific Expression Vector Harboring Gy1 Gene From Soybean and Transformation of Corn

Two seed-specificity expression vectors harboring seed storage protein Gy1 gene of soybean were constructed and the two vectors were transformed into embryogenic calluses of corn.Vector1,pCAMBIA1301-7αP-Gy1,was constructed by inserting Gy1 gene into pCAMBIA1301-7αP with selectable marker hyg,and vector 2,pCAMBIA1301-sbeⅡb-Gy1,was constructed by inserting Gy1 gene into pCAMBIA1301-BADH with salt-tolerance selectable marker BADH.The two vectors were transformed into embryogenic calluses of maizeH99mediated with agrobacterium.Thirty-four transgenic plants were confirmed by PCR analysis from 132 putative transgenic plants.