Characterisation of Streptozotocin Induced Diabetes Mellitus in Swiss Albino Mice

Abstract: The present study was designed to investigate the characteristics of diabetes mellitus induced by different doses of Streptozotocin in mice as assessed by blood glucose concentration. Swiss albino mice of either sex were intraperitoneally administered single dose of Streptozotocin 180 mg/kg, or 100 mg/kg and multiple low dose of Streptozotocin 40 mg/kg/day. A single injection of Streptozotocin 180 mg/kg was found to produce Type 1 (insulin-dependent diabetes mellitus) and multiple low dose Streptozotocin 40 mg/kg Type 2 (non-insulin-dependent diabetes mellitus). However, Streptozotocin 100 mg/kg failed to produce diabetes mellitus except a sustained hyperglycemia. Blood glucose concentrations were measured at every week after Streptozotocin injection. Multiple low doses of STZ 40 mg/kg, i.p. to mice for five consecutive days have been found suitable model to study long term complications of diabetes. Key words: Multiple low dose Mice Streptozotocin Diabetes mellitus INTRODUCTION different type in mice, the present study has Diabetes mellitus is the most common metabolic mellitus induced by administration of different doses of disorder. It is characterized by hyperglycemia that STZ. Therefore, to study the characteristic of diabetes results from an absolute or relative insulin deficiency mellitus three dose schedules were employed in the and is associated with long-term complications affecting present study.