微泡
接收机工作特性
细胞外小泡
小RNA
曲线下面积
2型糖尿病
生物标志物
曲线下面积
外体
1型糖尿病
2型糖尿病
医学
诊断生物标志物
糖尿病
诊断准确性
生物信息学
内科学
癌症研究
计算生物学
生物
化学
内分泌学
基因
遗传学
细胞生物学
生物化学
药代动力学
作者
Chunxing Liu,Yonghui Gao,WU Jian-fen,Jian Zou
出处
期刊:Clinical Laboratory
[Clinical Laboratory Publications]
日期:2021-01-01
卷期号:67 (02/2021)
被引量:6
标识
DOI:10.7754/clin.lab.2020.200612
摘要
Background Type 2 diabetes mellitus (T2D) is a common chronic disease, which lacks a specific diagnostic method. A substantial body of literature has demonstrated that the molecular constituents of extracellular vesicles (EVs) are promising as a novel biomarker for clinical diagnosis, especially exosomal miRNAs in biological fluids. Methods To find a diagnostic biomarker for T2D, we isolated exosomes from plasma and then quantitative PCR (qPCR) was used to detect the miRNA expression in plasma and exosomes from control subjects and T2D subjects. The ROC (receiver operating characteristic) curve and AUC (area under curve) were used to evaluate the diagnostic value of exosomal miRNAs. Results We found the exosomal levels of miR-23a and miR-192 were both significantly higher in T2D subjects. The AUC of miR-23a and miR-192 were 0.828 and 0.717, respectively. Further bioinformatics analysis was performed to speculate possible mechanisms. Conclusions In conclusion, exosomal miR-23a and miR-192 have good potential as diagnostic biomarkers for type 2 diabetes.
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