微流控
洗脱
试剂
高效液相色谱法
化学
制作
酶
材料科学
色谱法
纳米技术
生物化学
有机化学
医学
病理
替代医学
作者
Abraham Ochoa,Frida Trejo,Luis F. Olguín
出处
期刊:Methods in molecular biology
日期:2019-11-27
卷期号:: 209-233
被引量:4
标识
DOI:10.1007/978-1-0716-0163-1_14
摘要
Sub-nanoliter droplets produced in microfluidic devices have gained an enormous importance for performing all kinds of biochemical assays. One of the main reasons is that the amounts of reagents employed can be reduced in approximately five orders of magnitude compared to conventional microplate assays. In this chapter, we describe how to carry out the design, fabrication, and operation of a microfluidic device that allows performing enzyme kinetics and enzyme inhibition assays in droplets. This procedure can be used effectively to screen a small size library of compounds. Then, we describe how to use this droplet microfluidic setup to screen for potential inhibitor compounds eluted from a coupled high-performance liquid chromatography (HPLC) system that separates crude natural extracts.
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