轨道轨道
兽药
色谱法
兽药
甲酸
固相萃取
化学
萃取(化学)
质谱法
检出限
样品制备
高效液相色谱法
兽医学
医学
作者
Wentao Zhao,Rui Jiang,Wenping Guo,Chao Guo,Shilei Li,Juanqiang Wang,Shouwei Wang,Yingying Li
标识
DOI:10.1007/s00128-021-03273-w
摘要
A rapid, simple, and sensitive method of detecting veterinary drug residues in animal food sources, including poultry and pork, was developed and validated. The method was optimized for over 155 veterinary drugs of 21 different classes. Sample pretreatment included a simple solid-liquid extraction step with 0.2% formic acid-acetonitrile-water and a purification step with a PRiME HLB (hydrophile-lipophile balance) solid-phase extraction cartridge. Data were collected using ultra-high-performance liquid chromatography coupled to Quadrupole-Exactive Orbitrap mass spectrometry. The limits of detection of 155 veterinary drugs ranged from 0.1 µg/kg to 10 µg/kg. The recovery rates were between 79.2 and 118.5 % in all matrices studied, with relative standard deviation values less than 15% (n = 6). The evaluated method allows the reliable screening, quantification, and identification of 155 veterinary drug residues in animal source food and has been successfully applied in authentic samples.
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