化学
脂多糖
污渍
炎症
活性氧
超氧化物歧化酶
MAPK/ERK通路
生物化学
分子生物学
氧化应激
激酶
生物
免疫学
基因
作者
Mengqi Li,Dong Liu,Hanting Du,Zhijie Bao,Lei Qin
出处
期刊:Food Chemistry
[Elsevier]
日期:2021-08-01
卷期号:353: 129452-129452
被引量:32
标识
DOI:10.1016/j.foodchem.2021.129452
摘要
This study aimed to investigate the protective effects of a < 3 kDa Tricholoma matsutake Singer peptide (TMWP) on lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages. The results showed that TMWP significantly upregulated superoxide dismutase (SOD) activity and reduced reactive oxygen species (ROS) generation in RAW264.7 macrophages. Western blotting and immunofluorescence analysis indicated that TMWP inhibited the activation of the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways, thereby reducing the secretion of IL-1β and IL-6 and the expression of TNF-α, COX-2, and iNOS. Additionally, TMWP improved mitochondrial respiration in LPS-stressed macrophages, counteracting the harmful effects of LPS treatment on mitochondrial function. Three peptides (SDIKHFPF, SDLKHFPF, and WFNNAGP) with the highest predicted scores for potential anti-inflammatory activity were identified using nano-HPLC-MS/MS. These data indicated that T. matsutake peptides could be an attractive natural ingredient for developing novel functional foods.
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