玉米芯
纤维素酶
水解
生物转化
化学
酶水解
葡聚糖
木质纤维素生物量
生物量(生态学)
催化作用
金属有机骨架
产量(工程)
酶
β-葡萄糖苷酶
色谱法
有机化学
吸附
材料科学
生物
发酵
农学
冶金
原材料
作者
Tianran Zheng,Yang Luan,Meili Ding,Chen Huang,Jianfeng Yao
标识
DOI:10.1016/j.biortech.2021.126163
摘要
Metal-organic frameworks (MOFs) could serve as efficient matrixes to immobilize cellulase because of their high stability and porous morphology. Herein, the Zr-based MOFs (UiO-66 and UiO-66-NH2) assisted 20 wt% high-solids hydrolysis of untreated corncob residues (CRs) at low enzyme loading was investigated. Glucan hydrolysis yields increased to 60.55% and 71.47% by separately adding 4 g/L UiO-66 and UiO-66-NH2 at 5 FPU/g-glucan cellulase dosage. The maximum hydrolysis yield reached 90.01% at 10 FPU/g-glucan in the presence of 4 g/L UiO-66-NH2. Analysis of free protein concentration and cellulase activity suggested that MOFs effectively increased cellulase catalytic activity and stability, thus boosted CRs enzymatic hydrolysis efficiency. Additionally, UiO-66-NH2 immobilization gave a high catalytic activity because of the abundant anchor sites of NH2 groups. This research presents the promising future of MOFs' application in lignocellulosic biomass bioconversion and other areas requiring immobilized enzymes.
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