PLGA公司
组织纤溶酶原激活剂
聚乙二醇
溶栓
材料科学
PEG比率
纤溶酶原激活剂
碳二亚胺
纤溶剂
纳米医学
纳米颗粒
生物医学工程
化学
药理学
纳米技术
医学
有机化学
高分子化学
生物化学
心肌梗塞
财务
精神科
经济
作者
Masumeh Zamanlu,Morteza Eskandani,Jaleh Barar,Mehdi Jaymand,Parvin Samadi Pakchin,Mehdi Farhoudi
标识
DOI:10.1016/j.jddst.2019.101165
摘要
Conventional thrombolytic therapy of ischemic stroke by tPA shows sometimes complications or insufficient therapeutic effects due to limitations of tPA. To prolong the circulating time and the thrombolytic activity of tPA as well as augmenting the capacity of self-targeting of tPA, we aimed to engineer tPA-loaded biodegradable and biocompatible polymeric nanoparticles (NPs). The PEG (polyethylene glycol) attached to the poly(lactic-co-glycolic acid) (PLGA) through carbodiimide/N-hydroxysuccinimide chemistry and then the PEG-PLGA NPs containing tPA was formulated using single emulsion solvent diffusion/evaporation technique. The physicochemical and morphological properties of NPs and functional release profile were investigated and the thrombolytic activity of NPs was evaluated using in vitro models. The engineered NPs showed a smooth spherical shape with a mean size of 250–280 nm, PDI of 0.1–0.2, and zeta potential of −7 mV and −12 mV for PLGA and PEG-PLGA NPs, respectively. Drug entrapment efficiencies were 80–100% and the functional release studies showed decreasing the thrombolytic activity of NPs during the experiment time. Thrombolytic activity measurements revealed that the engineered NPs presented an enhanced thrombolytic activity compared to the plain tPA. Based on these findings, the tPA-PEG-PLGA NPs could be a robust nanomedicine for enhanced thrombolytic therapy.
科研通智能强力驱动
Strongly Powered by AbleSci AI