自愈水凝胶
视网膜
细胞生物学
再生(生物学)
生物物理学
视网膜
材料科学
3D生物打印
生物医学工程
组织工程
化学
生物
生物化学
神经科学
医学
高分子化学
作者
Pengrui Wang,Xin Li,Wei Zhu,Zheng Zhong,Amy Moran,Wenqiu Wang,Kang Zhang,Shaochen Chen
出处
期刊:Bioprinting
[Elsevier]
日期:2018-12-01
卷期号:12: e00029-e00029
被引量:26
标识
DOI:10.1016/j.bprint.2018.e00029
摘要
Recapitulating native retina environment is crucial in isolation and culturing of retina photoreceptors (PRs). To date, maturation of PRs remains incomprehensible in vitro. Here we present a strategy of integrating the physical and chemical signals through 3D-bioprinting of hyaluronic acid (HA) hydrogels and co-differentiation of retinal progenitor cells (RPCs) into PRs with the support of retinal-pigment epithelium (RPEs). To mimic the native environment during retinal development, we chemically altered the functionalization of HA hydrogels to match the compressive modulus of HA hydrogels with native retina. RPEs were incorporated in the culturing system to support the differentiation due to their regeneration capabilities. We found that HA with a specific functionalization can yield hydrogels with compressive modulus similar to native retina. This hydrogel is also suitable for 3D bioprinting of retina structure. The results from cell study indicated that derivation of PRs from RPCs was improved in the presence of RPEs.
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