snRNP公司
剪接体
RNA剪接
小核核糖核蛋白
拼接因子
核糖核蛋白
细胞生物学
生物发生
小核RNA
卡哈尔体
生物
核糖核酸
小剪接体
遗传学
化学
基因
非编码RNA
作者
Angela Krämer,Fabio Ferfoglia,Chia-Yu Huang,F. Mulhaupt,Dobrila Nešić,Goranka Tanačković
出处
期刊:Biochemical Society Transactions
[Portland Press]
日期:2005-06-01
卷期号:33 (3): 439-442
被引量:23
摘要
Human splicing factor SF3a is a part of the 17 S U2 snRNP (small nuclear ribonucleoprotein), which interacts with the pre-mRNA branch site early during spliceosome formation. The SF3a subunits of 60, 66 and 120 kDa are all required for SF3a function in vitro. Depletion of individual subunits from HeLa cells by RNA interference results in a global inhibition of splicing, indicating that SF3a is a constitutive splicing factor. Structure-function analyses have defined domains necessary for interactions within the SF3a heterotrimer, association with the U2 snRNP and spliceosome assembly. Studies aimed at the identification of regions in SF3a60 and SF3a66, required for proper intracellular localization, have led to a model for the final steps in U2 snRNP biogenesis and the proposal that SF3a is incorporated into the U2 snRNP in Cajal bodies.
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