生物
干细胞
造血
细胞生物学
造血干细胞
逆转录病毒
干细胞衰老理论
成体干细胞
造血干细胞移植
移植
免疫学
干细胞因子
体外
遗传学
内皮干细胞
基因
内科学
医学
作者
Ihor R. Lemischka,David H. Raulet,Richard C. Mulligan
出处
期刊:Cell
[Elsevier]
日期:1986-06-01
卷期号:45 (6): 917-927
被引量:859
标识
DOI:10.1016/0092-8674(86)90566-0
摘要
We have used retrovirus-mediated gene transfer to mark hematopoietic stem cells in vitro and have tracked the fate of these cells after their transplantation into lethally irradiated recipients. Several classes of stem cells are demonstrated, including cells whose progeny completely repopulate all hematopoietic lineages as well as cells that contribute predominantly to certain lineages or to specific anatomical locations. In a majority of recipients, we find that few (1 or 2) stem-cell clones account for the majority of the mature hematopoietic cells. These results coupled with retransplantation studies suggest an in vivo mechanism for the temporal control of stem-cell use. Further studies based on periodic sampling of primary recipients suggest that normal hematopoiesis results from the sequential activation of different stem-cell clones rather than from an averaged contribution of the entire stem-cell pool.
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