硫酸软骨蛋白多糖
生物
免疫金标记
细胞外基质
硫酸软骨素
单克隆抗体
蛋白多糖
分子生物学
链酶
细胞生物学
细胞培养
细胞
抗体
生物物理学
生物化学
糖胺聚糖
免疫学
胰蛋白酶
遗传学
酶
作者
Zafrira Avnur,Benjamin Geiger
出处
期刊:Cell
[Elsevier]
日期:1984-10-01
卷期号:38 (3): 811-822
被引量:260
标识
DOI:10.1016/0092-8674(84)90276-9
摘要
Chondroitin-sulfate containing proteoglycan (CSPG) of the extracellular matrix (ECM) was visualized in chick tissues and cell cultures with a monoclonal antibody, CS-56. Cultured cells of various origins contained dense punctate layers of CSPG on both the substrate and the cell surface, as determined by immunofluorescent and immunogold staining. Under culture conditions the CSPG-containing matrix was usually excluded from stable cell-to-substrate focal contacts. The substrate-attached CSPG exhibited remarkable chemical stability but could be successfully removed by pronase or chondroitinases ABC and AC. Incubation of living cells with CS-56 antibodies resulted in the clustering of surface CSPG into patches, indicating that the surface-bound CSPG is free to move laterally along the plasma membrane. The unique properties of the CSPG-containing ECM revealed by CS-56 antibodies and their relationships to specific types of cell contacts are discussed.
科研通智能强力驱动
Strongly Powered by AbleSci AI