Objective To establish the quality standard for Jiangzhijianfei Tablets(Radix Polygoni Multiflori,Radix Notoginseng and Radix Puerariae,etc.).Methods TLC was performed to identify Radix Polygoni Multiflori,Radix Notoginseng and Radix Puerariae.HPLC was used to determine the content of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside.Results Radix Polygoni Multiflor,Radix Notoginseng and Radix Puerariae could be identified by TLC.The quantitative method had the linear rangs of(0.38)μg~3.80μg.The average recovery was 99.91% and RSD was 1.5%.Conclusion The established method is simple,feasible and reproducible and can be used as the quality standards for Jiangzhijianfei Tablets.