Structure-Activity Relationships Study of Unsaturated Thioethers on Human Chronic Myelogenous Leukemia Cell K562

The structure-activity relationship of unsaturated thioethers related with growth inhibition on human chronic myelogenous leukemia cell K562 was explored.A library of unsaturated thioethers was prepared by parallel synthesis using the PEG catalyzed nucleophilic substitution reactions of unsaturated bromides and sulfides.The inhibition effect of these unsaturated thioethers on proliferation of K562 cell was conveniendy determined upon the products directly in 96-well microtiter test plates by the commercial available MTT(tetrazolium salt) assay.This method revealed the diallyl trisulfide skeleton exhibited the highest activity:67%of inhibition ratio under 25μM.Structure-activity relationships of unsaturated thioethers related with growth inhibition on human chronic myelogenous leukemia cell K562 were constructed by comparing activities.