Simultaneous fluorescence determination of D,L-tryptophan enantiomer by chiral recognition of β-cyclodextrin

Objective:To accomplish simultaneous fluorescence determination of D,L _tryptophan enantiomer by chiral recognition of β_cyclodextrin.Methods:Chiral discrimination was observed for fluorescence emission of D _ and L _tryptophan when complexed to β_Cyclodextrin, and was significantly related to temperature, time and pH of reaction. The proposed enantiomeric resolution of D _ and L _tryptophan is based on the non_fluorescence emission of L_tryptophan in β_cyclodextrin solution under 35℃ and after 24*!h standing by.Results and Conclusion:The method was shown to be accurate with a ratio of 2%(mol/mol) of one enantiomer in the presence of the other.