Expression of C-X-C Motif Chemokine 10 and Interferon-γ in Fibrosis Lung Tissue of Silica Exposed Rats

[Objective]To compare the expression of C-X-C motif chemokine 10(CXCL10) and interferon-γ(IFN-γ) in lung tissue of experimental silicosis rats,and to provide a new way for study on pathogenesis of silicosis.[Methods]Twelve healthy male SD rats were divided into an experimental group and a control group which were administered with silica and saline respectively by non-exposed tracheal perfusion.A silicosis rat model was successfully established after 40 days of exposure and confirmed by pathological observation.Gene and protein expressions of CXCL10 and IFN-γwere detected and compared between the two groups by real-time quantitative polymerase chain reaction and immunohistochemistry,respectively.[Results]Compared with the control group,the gene expressions of CXCL10 and IFN-γin the silicosis group showed no significant difference.However,the protein expressions of CXCL10 and IFN-γof the silicosis group were remarkably higher than those of the control group(P0.05).[Conclusion]CXCL10 and IFN-γexpressions in silicosis tissue are higher than those in normal lung tissue,indicating that these 2 cytokines may play an important role in the development of pulmonary fibrosis.