Objective: To establish thin layer chromatography(TLC) method for identification of Zijintougu spray.Methods: Radix ceratostigmae,dragon's blood,Rhizoma bletillae,Gaultheria yunnanensis,turpentine and borneolum in Zijntougu spray were indentified by TLC method.Results: In the TLC,these six medicinal materials had characteristic spots,There were no corresponding spots at the same site in negative sample.Conclusions: TLC is simple,reliable with strong specificity and good reproducibility for quality control of Zijintougu spray.